| 谭军,秦晓东,欧阳华伟,李波.调Q1064nm 激光联合皮肤原位再生复原技术治疗小鼠黄褐斑模型的实验研究[J].中国烧伤创疡杂志,2012,24(6):423~431. |
| DOI：10.3969/j.issn.1001-0726.2012.6.001 |
| 中文关键词: 黄褐斑 调Q1064nm 激光 皮肤原位再生复原技术 酪氨酸酶 实验研究 |
| 英文关键词:Chloasma Q1064nm switched laser In situ skin regenerative restoration technique Tyrosinase Experimental study |
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| 中文摘要: |
| 目的 探讨调Q1064nm 激光联合皮肤原位再生复原技术对小鼠黄褐斑模型黑素细胞及酪氨酸酶表达的影响。方法 采用紫外线照射联合雌激素注射建立小鼠黄褐斑动物实验模型, 选择调Q1064nm 激光联合皮肤原位再生复原技术治疗, 修复其创面, 利用免疫组化方法分别检测调Q1064nm 激光联合皮肤原位再生复原技术作用前后小鼠皮肤皮损区域黑色素的变化及酪氨酸酶的表达水平, 并与对照组及正常组进行比较。结果 与对照组相比, 激光联合湿润烧伤膏(MEBO) 治疗组皮损区黑色素的表达明显降低(P <0． 05), 酪氨酸酶的表达水平明显下降, 与其他对照组相比, 有显著性差异(P <0． 05)。结论 调Q1064nm 激光干预治疗后, 通过湿润烧伤膏外涂为创面创造一个再生的生理湿润环境, 调节黄褐斑皮损区的局部环境, 减少酪氨酸酶的表达, 使黑色素的生成减少, 从而抑制黄褐斑的产生。 |
| 英文摘要: |
| Objective To explore the effect of Q1064nm switched laser combined with in situ skin regenerative
restoration technique on the expression of melanocyte and tyrosinase of mouse model of Chloasma. Methods Establish
mouse Chloasma models by injecting progesterone and exposing to UVB. Select Q1064nm switched laser in combination with
in situ skin regenerative restoration technique to treat and repair the wounds. Immunohistochemical technique was utilized to
detect the changes of melanin and the expression of tyrosinase at the mouse skin lesions before and after the treatment, which
was compared with the control group and the normal group. Results Compared with the other two groups, the expressions
of melanin and tyrosinase in the experimental group decreased sharply, which had significant difference (P <0． 05 respectively).
Conclusion The topical application of MEBO on the experimental zones after the intervention treatment of
Q1064nm switched laser can maintain a moist physiological environment for wound regeneration, regulate the local environment
of Chloasma sites, reduce the expression of tyrosinase and the production of melanin and finally inhibit the development
of Chloasma. |
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