| 唐乾利,付军,韩珊珊,李杰辉,狄钾骐,代波,冯婧,李利青,何晓薇.MEBT/ MEBO 对大鼠慢性难愈性皮肤溃疡创面肉芽组织EGF、EGFR 表达的影响[J].中国烧伤创疡杂志,2012,24(6):470~480. |
| DOI：10.3969/j.issn.1001-0726.2012.6.013 |
| 中文关键词: MEBT/ MEBO 难愈性皮肤溃疡 EGF EGFR 实验研究 |
| 英文关键词:MEBT/ MEBO Refractory skin ulcer EGF EGFR Experimental study |
| 基金项目:2008 年国家自然科学基金项目(№: 30860356) |
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| 中文摘要: |
| 目的 探讨烧伤湿性医疗技术(MEBT/ MEBO) 及配套产品湿润烧伤膏(MEBO) 对大鼠慢性难愈性皮肤溃疡创面肉芽组织表皮生长因子(EGF)、表皮细胞生长因子受体(EGFR) 表达的影响。方法 SPF 级SD 雄性大鼠45 只, 参照付小兵大鼠慢性性皮肤溃疡创面模型制作方法制作模型, 成模后再采用随机数字法随机分为模型组(15 只)、MEBO 组(15 只)、贝复剂组(15 只), 造模后第2 天开始用药, 观察用药后各组创面愈合情况、肉芽组织生长情况, 记录创面愈合时间; 常规病理切片观察创面炎症、成纤维细胞数量、血管改变情况; 透射电镜观察成纤维细胞及其线粒体形态结构; 换药后第7、14 天取创面组织, 采用免疫组织化学法(SP法) 观察MEBO 对创面肉芽组织EGF、EGFR 表达的影响。结果 MEBO 组大鼠的创面愈合情况明显好于模型组与贝复济组; 创面愈合时间明显短于模型组及贝复济组(P <0． 05; P <0． 01); 病理学观察: MEBO 组与贝复剂组及模型组比较, 炎症反应轻、肉芽组织生长良好, 纤维增生程度低; 换药后第7、14 天, MEBO 组大鼠创面肉芽组织中EGF 和EGFR 蛋白表达阳性染色指数分别较模型组和贝复济组大鼠创面肉芽组织中EGF 和EGFR 蛋白表达阳性染色指数提高, 差异有统计学意义(P < 0． 05)。结论 MEBO 能增加慢性皮肤溃疡创面肉芽组织中EGF、EGFR 的表达量, 从而促进慢性皮肤溃疡愈合。 |
| 英文摘要: |
| Objective To explore the effects of MEBT/ MEBO (i． e. Moist Exposed Burn Therapy and Moist Exposed
Burn Ointment) on the expression of epidermal growth factor (EGF) and epidermal growth factor receptor (EGFR) in
the wound granulation tissue of rat chronic skin ulcer. Methods Select 45 SPF SD male rats to make the rat chronic skinulcerative wound models referring to the modeling method of FU Xiao-bin (Reference [1]). After the formation of the model,
randomly divide the 45 rats into three groups: model group (15 rats), MEBO group (15 rats) and rb-bFGF (Recombinant
Bovine Basic Fibroblast Growth Factor for External Use) group (15 rats). From the second day after the model formation,
begin to apply the medications on the wounds. The wound healing situation and the growth situation of granulation were
observed, and the wound healing time was recorded. Take routine pathological section to observe the inflammatory situation,
amount of fibroblasts, changes of blood vessels. Use transmission electron microscope to observe the morphology and structure
of fibroblasts and their mitochondria. At the seventh and fourteenth days of dressing change, take the wound tissue and
observe the effect of MEBO on the expression of EGF and EGFR in granulation tissue by using the Immunohistochemical
method (SP method). Results The healing situation and the healing time in MEBO group were both superior to that in the
other two groups (P <0． 05, P <0． 01). Pathological observation results: compared with the other two groups, MEBO group
showed slighter inflammation, much better growth of granulation tissue and slighter fibroplasia. At the seventh and fourteenth
days of dressing change, the positive staining index of EGF and EGFR in the granulation tissue of MEBO group was higher
than that in the other two groups, and the difference between them was significant in statistics (P <0． 05). Conclusion
MEBO can increase the expression of EGF and EGFR in granulation tissue of chronic skin ulcerative wound and thereby promote
the healing of chronic skin ulcer. |
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