| 刘沂霖,李利平,陈季玲,郑军,周昌宁,严磊,周艳丹,侯玉洁,刘奇.大鼠皮肤急性深Ⅱ度β射线损伤创面愈合过程中的对比观察[J].中国烧伤创疡杂志,2014,(1):30~35. |
| DOI： |
| 中文关键词: 损伤 烫伤 创面 β射线 β１ 整合素 |
| 英文关键词:Injury Scald Wound β-ray Integrin β1 |
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| 中文摘要: |
| 目的 观察大鼠皮肤急性深Ⅱ度β射线损伤创面自然愈合过程中β1 整合素表达阳性细胞的动态变化, 探讨急性β射线皮肤损伤创面的难愈机制? 方法 将96只SD大鼠随机分为实验组(48只) 与对照组(48只), 实验组建立大鼠皮肤急性深Ⅱ度β射线损伤创面模型; 对照组建立大鼠皮肤深Ⅱ度热力烫伤创面模型?分别于伤后1d? 3d? 7d? 14d? 21d? 28d? 35d记录创面变化, 并取创面组织标本, 行HE染色和免疫组化染色, 观察创面愈合过程中的组织变化和β1 整合素阳性细胞的变化? 结果 实验组大鼠创面愈合时间较对照组长,经统计学分析, 差异有统计学意义(P<0.01); 通过对β1 整合素的检测发现, 照射及烫伤后1 d? 3 d? 7 d?14d? 21d的β1 整合素阳性细胞IOD值比较, 差异有统计学意义(P<0.05), 对照射及烫伤后28d? 35d的β1整合素阳性细胞IOD值进行比较, 差异无统计学意义(P>0.05)? 结论 皮肤急性深Ⅱ度β射线损伤创面愈合时间相对缓慢, 且在愈合过程中β1 整合素阳性细胞增殖分化延迟, 可能是皮肤急性深Ⅱ度β射线损伤创面延迟愈合的原因之一? |
| 英文摘要: |
| Objective: To investigate the healing mechanisms of refractory acute β-ray-injured skin wound by observing the dynamic changes of cells expressing integrin β1 during the natural healing process of acute β-ray-injured deep second degree skin wound in rats. Methods: 96 SD rats were randomly divided into an experimental group (n=48) and a control group (n=48). Build a rat model of acute deep second-degree skin wound caused by β-ray in the experimental group; set up a rat model of deep second-degree skin wound of thermal scald in the control group. On 1d, 3d, 7d, 14d, 21d, 28d, 35d post-injury, record the wound changes and take wound tissue sample for HE staining and immunohistochemical staining to observe the histological changes and changes of cells expressing integrin β1 during the process of wound healing.Results: Compared with the control group, the wound healing time of rats in the experimental group was longer and the difference was significant according to the statistical analysis (P<0.01). The detection of integrin β1 showed there was a statistically significant difference between the two groups in terms of IOD value of cells expressing integrin β1 on day 1, day 3, day 7, day 14, day 21 post-injury (P<0.05), but the IOD value on day 28 and day 35 showed no statistically significant difference between the two groups (P＞0.05). Conclusion: The healing time of acute deep-second degree skin wound caused by β-ray was relatively longer and the proliferation and differentiation of cells expressing integrin β1 were delayed during the healing process which could be one of the reasons why the healing of β-ray-injured acute deep-second degree skin wounds was delayed. |
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