| 唐乾利,李利青,何晓微,黄 欣,王 兵,吕 震,金 萌,李 辉,王 宇.MEBT/MEBO对糖尿病足溃疡创面愈合中Smad3、β-catenin mRNA表达的影响[J].中国烧伤创疡杂志,2014,(5):331~341. |
| DOI： |
| 中文关键词: 皮肤原位再生复原技术 β-catenin Smad3 mRNA 糖尿病足溃疡 创面愈合 |
| 英文关键词:Skin In Situ Regenerative Restoration Technology (MEBT/MEBO) β-catenin Smad3 mRNA Diabetic Foot Ulcer(DFU) Wound Healing |
| 基金项目:2013年广西自然科学基金重点项目（项目批准号：2013GXNSFDA019020） |
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| 中文摘要: |
| 目的 采用RT-PCR检测方法观察皮肤原位再生复原技术（MEBT/MEBO）对大鼠糖尿病足溃疡创面愈合的干预作用，研究其促进溃疡创面愈合的机制。方法 将100只雄性SD大鼠随机分为空白对照组（20只）和糖尿病造模组（80只），糖尿病造模组大鼠尾静脉注射链脲佐菌素（STZ），成功制备糖尿病大鼠模型64只，从中随机选取60只，并随机分为湿润烧伤膏（MEBO）组（20只）、贝复济组（20只）和模型组（20只）。造模成功后次日开始用药，各组大鼠创面给予相应的药物治疗，并记录创面愈合时间；用药6 d后，采用RT-PCR法观察记录创面肉芽组织中β-catenin mRNA和Smad3 mRNA的表达量。结果 （1）4组大鼠创面平均愈合时间对比，空白对照组愈合时间最短；MEBO组、贝复济组、模型组3组大鼠创面平均愈合时间对比，MEBO组愈合时间最短。（2）用药后第6天，模型组大鼠创面肉芽组织中β-catenin mRNA的表达显著低于空白对照组、MEBO组、贝复济组（P＜0.01），而MEBO组与贝复济组相比，差异具有统计学意义（P<0.05）；模型组大鼠创面肉芽组织中Smad3 mRNA的表达显著高于空白对照组、MEBO组、贝复济组（P<0.01），而MEBO组与贝复济组相比，差异具有统计学意义（P<0.05）。结论 MEBT/MEBO可抑制Smad3 mRNA、增强β-catenin mRNA在实验性糖尿病足溃疡大鼠创面修复中的表达，缩短创面愈合时间，促进糖尿病足溃疡创面的愈合。 |
| 英文摘要: |
| Objective To study the mechanism of MEBT/MEBO promoting the healing of diabetic foot ulcer (DFU) wounds by inspecting the intervention of MEBT/MEBO in the wound healing of rat DFU through RT-PCR detection. Methods One hundred male SD rats were divided randomly into blank Control Group (20) and Diabetes Model Building Group (80), the latter one was injected with STZ in caudal vein, 64 rat models were successfully built, and 60 of them were randomly selected and divided into MEBO group (20), Rb-bFGF group (20) and model group (20). After the model was built, the medicine was administered the next day, each group of rat wounds was given corresponding medical treatment and the healing time was recorded. Six days later, RT-PCR was adopted to detect and record the amount of β-catenin mRNA and Smad3 mRNA expression in granulation tissues of wounds. Results (1) Comparing the average healing time of the 4 groups of rats, the blank Control Group was the shortest; The average healing time for MEBO Group was the shortest compared with Rb-bFGF Group and Model Group. (2) Six days after drug administration, the expression of β-catenin mRNA in wound granulations of rats in Model Group was obviously lower than that of blank Control Group, MEBO Group, and Rb-bFGF Group (P＜0.01), and comparison between MEBO Group and Rb-bFGF group showed difference of statistical significance (P＜0.05); The expression of Smad3 mRNA in wound granulations of rats in Model Group was obviously higher than blank Control Group, MEBO Group, and Rb-bFGF Group (P＜0.01), and comparison between MEBO Group and Rb-bFGF group showed difference of statistical significance (P＜0.05). Conclusion MEBT/MEBO can repress expression of Smad3 mRNA and enhance expression of β-catenin mRNA in healing of experimental DFU wounds of rats, shortening wound healing time and promoting healing of DFU wounds. |
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