Objective To investigate the standards of clinical management and treatment of Wagner 3-5 diabetic foot wounds with limb salvation and terminating amputation as the end points. Methods Based on the experience of healing 102 patients with Wagner 3-5 diabetic foot wounds by using MEBO in combination with surgical debridement, summarize the standards of clinical management and treatment of Wagner 3-5 diabetic foot wounds. Results The clinical evidence from the healed 102 cases with Wagner 3-5 diabetic foot wounds has sufficiently demonstrated that significant therapeutic effects can be achieved when MEBO in combination with surgical debridement is applied to treat Wagner 3-5 diabetic foot wounds. Conclusion MEBO in combination with surgical debridement can be the standard of managing and treating Wagner 3-5 diabetic foot wounds in clinic with its strength of limb salvation and terminating amputation.