| 葛 斌,李利青,唐乾利,曾鸿孟,王 澍,单云龙,陈端凯.皮肤再生医疗技术对大鼠慢性难愈合创面组织中VEGFR2表达的动态调控作用[J].中国烧伤创疡杂志,2017,(6):399~405. |
| DOI： |
| 中文关键词: 慢性难愈合创面 皮肤再生医疗技术 湿润烧伤膏 VEGFR-2 动态调控 实验研究 |
| 英文关键词:Chronic and hard-to-healing wounds Skin regenerative medical technology Moist exposed burn ointment VEGFR-2 Dynamic regulation Experimental research |
| 基金项目:2017 年中国红十字基金会徐荣祥再生生命公益基金科研项目(项目编号: 中国红基会[2017] 188 号); 国家自然科学基金项目(项目编号: 81560776) |
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| 中文摘要: |
| 目的 动态观察皮肤再生医疗技术(moist exposed burn therapy/moist exposed burn ointment, MEBT/MEBO) 对大鼠慢性难愈合创面组织中VEGFR-2 表达水平的影响, 探讨MEBT/ MEBO 促进创面愈合的分子学机制?方法 按照随机数表法将90 只健康成年SPF 级SD 雄性大鼠随机分为空白组(18 只)?对照组(18 只)?模型组(18 只)?MEBO 组(18 只) 和贝复济组(18 只), 其中空白组仅做备皮处理, 对照组做全层皮肤缺损创面模型, 模型组?MEBO 组和贝复济组做慢性难愈合创面模型; 模型建立后, 空白组大鼠备皮后皮肤予以生理盐水纱布湿敷, 对照组及模型组大鼠创面予以生理盐水纱布覆盖治疗, MEBO 组大鼠创面予以湿润烧伤膏(moistexposed burn ointment, MEBO) 药纱覆盖治疗, 贝复济组大鼠创面予以贝复济药纱覆盖治疗, 并分别于治疗后第3?7?14 天检测?对比各组大鼠创面组织中VEGFR-2 蛋白及VEGFR-2 mRNA 的表达水平?结果 创面处理后第3?7?14 天, 空白组大鼠皮肤组织中VEGFR-2 蛋白及VEGFR-2 mRNA表达水平均无明显变化, P 均> 0.05, 差异无统计学意义; 而对照组?模型组?MEBO 组及贝复济组大鼠创面组织中VEGFR-2 蛋白及VEGFR-2 mRNA 表达水平变化均较明显, P 均< 0.05, 差异具有统计学意义, 其中对照组?MEBO 组及贝复济组大鼠创面组织中VEGFR-2 蛋白及VEGFR-2 mRNA 表达水平均呈先升高后降低的趋势, 而模型组则呈递增趋势?治疗第3?7?14天, MEBO组大鼠创面组织中VEGFR-2 蛋白和VEGFR-2 mRNA 表达水平均高于模型组, P 均<0.05, 差异具有统计学意义?结论 MEBT/MEBO 可通过上调创面组织中VEGFR-2 的表达水平, 促进创面愈合? |
| 英文摘要: |
| Objective To have a dynamic observation of the influence of MEBT/MEBO on the expression level of VEGFR-2 in rat chronic and hard-to-healing wound tissues and explore the molecular mechanism of MEBT/MEBO promoting wound healing. Methods 90 healthy SPF SD male rats were randomly divided into five groups: blank group (18), control group (18), model group (18), MEBO group (18), BFGF group (18). For blank group, only skin preparation was performed; for control group, the full thickness skin defect model was established; for model group, MEBO group and BFGF group, chronic and hard-to-healing wound models were established. After the models were established, the prepared skin of rats in blank group was dressed with normal saline gauze; rats’ wounds of control group and model group were covered with normal saline gauze; rats’ wounds of MEBO group were treated with MEBO gauze; rats’ wounds of BFGF group were treated with BFGF gauze. The expression level of protein VEGFR-2 and VEGFR-2 mRNA in rats’ wounds of the above groups were determined and compared after 3, 7 and 14 days of treatment respectively. Result On day 3, day 7 and day14 after wound management, no significant change was observed in the expression level of protein VEGFR-2 and VEGFR-2 mRNA in rats’skin tissues of blank group (P＞0.05, no statistically significant difference).Significant changes were observed in the expression level of protein VEGFR-2 and VEGFR-2 mRNA in rats’wound tissues of control group, model group, MEBO group and BFGF group and the difference was statistically significant (P＜0.05).In control group, MEBO group and BFGF group, expression level of protein VEGFR-2 and VEGFR-2 mRNA increased at first and decreased after a period of time, while it kept increasing all the time in model group. On days 3，7 and 14 of treatment, expression level of protein VEGFR-2 and VEGFR-2 mRNA in rats’wounds of MEBO group was higher than model group and the comparison showed statistically significant difference(P＜0.05). Conclusion MEBT/MEBO can increase the expression level of VEGFR-2 in wound tissues, hence promoting wound healing. |
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