| 黄桃源,何仁亮.伊曲康唑对皮肤鳞状细胞癌A431细胞抑制作用的初步研究[J].中国烧伤创疡杂志,2020,(1):46~51. |
| DOI： |
| 中文关键词: 伊曲康唑 皮肤 鳞状细胞癌 抑制 血管内皮生长因子 研究 |
| 英文关键词:Itraconazole Skin Squamous cell carcinoma Inhibition Vascular endothelial growth factor Research |
| 基金项目:广东省医学科学技术基础研究基金项目（B2016015） |
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| 中文摘要: |
| 【摘要】目的 研究伊曲康唑（itraconazole，ITZ）对皮肤鳞状细胞癌（cutaneous squamous cell carcinoma，CSCC）A431细胞的生物学效应，探讨其对皮肤鳞状细胞癌的抑制机制。方法 取对数生长期A431细胞随机分为2、5、10、15、20、25及30 μg/mL浓度组与对照组，依据分组分别采用等体积2、5、10、15、20、25及30 μg/mL浓度ITZ溶液及DMEM培养基对细胞进行处理，并分别采用MTT法、流式细胞仪检测法、RT-PCR法和Western blotting法检测各组A431细胞的增殖情况、细胞周期及血管内皮生长因子（VEGF）mRNA及蛋白的表达水平。结果 随着ITZ浓度的增加及作用时间的增长，ITZ对A431细胞的抑制作用逐渐增强，且除培养24 h时2 μg/mL组细胞生长抑制率与对照组无明显差异（P＞0.05）外，培养24 h时5、10、15、20、25、30 μg/mL组细胞生长抑制率及培养48、72 h时2、5、10、15、20、25、30 μg/mL组细胞生长抑制率分别与对照组对比，P均＜0.05，差异具有统计学意义；与对照组相比，经浓度分别为2、5、10、15、20、25及30 μg/mL的ITZ处理后，A431细胞的G0/G1期均出现延长，且除15 μg/mL组与对照组无明显差异（P＞0.05）外，其余各组与对照组对比，P均＜0.05，差异具有统计学意义；与对照组相比，除ITZ浓度为10 μg/mL时，VEGF mRNA及蛋白的表达水平明显降低（P均＜0.05）外，ITZ浓度为2、5、15、20、25及30 μg/mL时，VEGF mRNA及蛋白的表达水平均明显升高（P均＜0.05）。结论 ITZ能够抑制CSCC A431细胞的增殖并延长细胞周期，然而其对VEGF的影响却没有规律性，故其抑制细胞增殖的具体机制仍需进一步深入研究。 |
| 英文摘要: |
| 【Abstract】Objective To study the biological effect of itraconazole (ITZ) on A431 cells in cutaneous squamous cell carcinoma (CSCC) and explore its inhibitory mechanism on cutaneous squamous cell carcinoma (CSCC). Methods A431 cells at logarithmic growth phase were taken and then randomly divided into concentration groups at the concentration of 2, 5, 10, 15, 20, 25, and 30 μg / mL and a control group. The cells in these groups were treated with equal volumes of ITZ solution at the concentration of 2, 5, 10, 15, 20, 25, 30 μg / mL and DMEM medium respectively. MTT assay, flow?cytometry, RT-PCR and Western blotting method were used to detect the proliferation and cell cycle of A431 cells and the expression of vascular endothelial growth factor (VEGF) mRNA and protein in each group. Results As the concentration of ITZ and its duration of action increased, its inhibitory effect on A431 cells improved gradually. No significant difference in cell growth inhibition rate was observed at 24th hour of culture between the 2 μg/m group and the control group (P>0.05); cell growth inhibition rate of 5, 10, 15, 20, 25, 30 μg / mL groups at 24th hour of culture and cell growth inhibition rate of 2, 5, 10, 15, 20, 25, 30 μg / mL groups at 48th and 72nd hour of culture were compared with those of the control group respectively (all P<0.05) and the results of comparison showed statistically significant difference; after treatment with ITZ at concentrations of 2, 5, 10, 15, 20, 25, and 30 μg / mL, the G0 / G1 phase of A431 cells were prolonged in the concentration group compared with that in the control group; no significant difference was observed between the 15 μg/mL group and the control group (P>0.05), and the results of comparison between the rest concentration groups and the control group showed statistically significant difference (all P<0.05); compared with the control group, only when the ITZ concentration was 10 μg / mL, the expression levels of VEGF mRNA and protein decreased significantly (all P <0.05), and when the ITZ concentration was 2, 5, 15, 20, 25, and 30 μg / mL, the expression levels of VEGF mRNA and protein increased significantly (all P <0.05).Conclusion ITZ can inhibit the proliferation and prolong the cell cycle of CSCC A431 cells. However, its effect on VEGF remains unpredictable. Therefore, the specific mechanism of its inhibition on cell proliferation needs further research. |
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