| 任晓燕,王李雯,张丽丽,孙丽萍.湿润烧伤膏对寻常型银屑病小鼠IL23/Th17轴及其相关因子表达水平的影响[J].中国烧伤创疡杂志,2020,(3):153~161. |
| DOI： |
| 中文关键词: 湿润烧伤膏 寻常型银屑病 炎症因子 IL23/Th17轴 作用机制 |
| 英文关键词:MEBO Psoriasis vulgaris Inflammatory factors IL23 / Th17 axis Mechanism of action |
| 基金项目:中国红十字基金会徐荣祥再生生命公益基金科研项目(2018-01) |
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| 中文摘要: |
| 目的 观察湿润烧伤膏（moist exposed burn ointment，MEBO）对咪喹莫特（Imiquimod，IMQ）诱导的寻常型银屑病小鼠皮损组织中白细胞介素-17（IL-17）、白细胞介素-23（IL-23）、肿瘤坏死因子-α（TNF-α）及干扰素-γ（IFN-γ）水平的影响，并探讨其作用机制。方法 选取40只SPF级健康雌性6～7周龄近交系BALB/c小鼠建立咪喹莫特诱导的寻常型银屑病模型，模型建立成功后采用随机数表法将35只小鼠随机分为对照组、模型组、MEBO低剂量组、MEBO中剂量组及MEBO高剂量组，并分别采用卡泊三醇乳膏、麻油、1:3湿润烧伤膏与麻油混合物、1:1湿润烧伤膏与麻油混合物、湿润烧伤膏处理皮损创面，治疗第7、14天参照银屑病皮损面积与严重程度指数（psoriasis area and severity index，PASI）评分标准评估5组小鼠皮损严重程度，治疗第14、28天采用Western blotting法检测IL-17、IL-23、TNF-α及IFN-γ蛋白表达水平，并予以对比。结果 治疗第7天，对照组、MEBO中剂量组及MEBO高剂量组小鼠背部皮损PASI评分均显著低于模型组(P均＜0.05），且对照组、MEBO中剂量组及MEBO高剂量组组间两两对比，PASI评分均无明显差异（P均＞0.05）；治疗第14天，5组小鼠皮损症状均明显改善，PASI评分无明显差异（P＞0.05）；治疗第14天，小鼠背部皮损组织中IL-17、IL-23、TNF-α及IFN-γ蛋白表达水平对比，对照组显著低于其他各组（P均＜0.05），且MEBO高剂量组显著低于模型组（P均＜0.05)；治疗第28天，小鼠背部皮损组织中IL-17、IL-23、TNF-α及IFN-γ蛋白表达水平对比，对照组显著低于模型组、MEBO低剂量组及MEBO中剂量组（P均＜0.05），与MEBO高剂量组无明显差异（P均＞0.05）；MEBO高剂量组和MEBO中剂量组显著低于模型组和MEBO低剂量组（P均＜0.05)，且MEBO高剂量组与MEBO中剂量组无明显差异(P均＞0.05)。结论 湿润烧伤膏治疗银屑病的疗效与卡泊三醇软膏相当，且调节IL-23/Th17轴及其相关因子的表达水平，抑制炎症反应可能是其作用机制。 |
| 英文摘要: |
| 【Abstract】 Objective To observe the effect of MEBO on the levels of interleukin-17 ( IL-17), interleukin-23 (IL-23), tumor necrosis factor-α (TNF-α) and interferon-γ (IFN-γ) in mice with psoriasis vulgoriasis induced by Imiquimod (IMQ) and explore its mechanism of action. Methods Forty 6-to 7-week-old specific-pathogen free ( SPF) healthy inbred BALB/ c female mice were selected and imiquimodinduced psoriasis vulgaris models were established on them. After the models were established, 35 of them were randomly divided into a control group, a model group, a MEBO low-dose group, a MEBO mediumdose group and a MEBO high-dose group and their skin lesions were treated with calcipot-riol cream, sesame oil, mixture of MEBO and sesame oil at the ratio of 1∶ 3, mixture of MEBO and sesame oil at the ratio of 1∶ 1 and MEBO respectively. On day 7 and 14 of treatment, the severity of skin lesions of the five groups of mice were eval-uated based on psoriasis area and severity index (PASI) scoring system. On day 14 and 28 of treatment, Western blotting was used to detect the protein expression levels of IL-17, IL-23 TNF-α and IFN-γ, which were then compared among each other. Results On day 7 of treatment, the PASI scores of the skin lesions on the backs of mice in the control group, the MEBO medium-dose group and the MEBO high-dose group were significantly lower than the model group and MEBO low-dose group (all P < 0.05). Pairwise comparisons in PASI scores were made among the control group, the MEBO medi-um-dose group and the MEBO high-dose group and the results (all P > 0.05) showed no significant difference; on day 14 of treatment, the skin lesions on the backs of the five groups of mice improved significantly and no significant difference was observed in PASI scores (P > 0.05); on day 14 of treatment, the protein expression levels of IL-17, IL-23, TNF-α and IFN-γ in skin tissues on the backs of the mice were compared: their levels in the control group was significantly lower than other groups (all P < 0.05) and their levels in the MEBO high-dose group was significantly lower than the model group (all P < 0.05); on day 28 of treatment, the protein expression levels of IL-17, IL-23, TNF-α and IFN-γ in the skin tissues on back of the mice were compared: their levels in the control group was significantly lower than the model group, the MEBO low-dose group and the MEBO medium-dose group ( all P < 0.05) and no significant difference was observed between the levels of the control group and MEBO high-dose group (all P > 0.05); their levels in the MEBO high-dose group and the MEBO medium-dose group were significantly lower than the model group and the MEBO low-dose group ( all P < 0.05), and no significant difference was observed between the MEBO high-dose group and the MEBO medium-dose group (all P > 0.05). Conclusion MEBO has similar effect with calcipotriol cream in treating psoriasis. Their mechanism of action may lie in their ability to regulate the expression of IL-23 / Th17 axis and its related factors, and inhibit inflammatory reactions. |
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