| 唐乾利,舒清峰,单云龙,李利青,唐 强,岑小宁,唐习强,许 彦,黄丽芳.MEBT/MEBO对慢性难愈合创面组织中CK14及CK19表达水平的影响[J].中国烧伤创疡杂志,2020,(4):229~239. |
| DOI： |
| 中文关键词: 皮肤再生医疗技术 重组牛碱性成纤维细胞生长因子 慢性难愈合创面 细胞角蛋白14 细胞角蛋白19 |
| 英文关键词:MEBT/MEBO Recombinant bovine basic fibroblast growth factor Chronic refractory wound Cytok⁃ eratin 14 Cytokeratin 19 |
| 基金项目:国家自然科学基金面上项目 (81774327); “广西特聘专家” 专项经费资助项目 (桂人才通字 [2019] 13 号); 广西 医学高层次骨干人才培养 “139” 计划项目资助 (桂卫科教发 [2018] 22 号) |
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| 摘要点击次数: 2013 |
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| 中文摘要: |
| 目的 探讨皮肤再生医疗技术（moist exposed burn therapy/moist exposed burn ointment，MEBT/MEBO）对大鼠慢性难愈合创面组织中CK14及CK19表达水平的影响。方法 按照随机数表法将90只SPF级Wistar大鼠随机分为空白组、急创组、慢创组、rb-bFGF组及MEBO组，每组18只，空白组大鼠仅做背部备皮处理，急创组大鼠建立急性创面模型，慢创组、rb-bFGF组及MEBO组大鼠建立慢性创面模型；造模成功后，空白组大鼠备皮处皮肤以及急创组与慢创组大鼠创面予以生理盐水纱布湿敷，rb-bFGF组大鼠创面予以重组牛碱性成纤维细胞生长因子（recombinant bovine basic fibroblast growth factor，rb-bFGF）凝胶治疗，MEBO组大鼠创面予以湿润烧伤膏（moist exposed burn ointment，MEBO）治疗。分别于局部处理第3、7、14天取各组大鼠皮肤或创面组织行Masson染色，观察组织形态学差异，并进行Western blotting及RT-PCR检测，对比CK14、CK19蛋白表达及其mRNA转录水平。结果 （1）Masson染色结果显示，急创组、rb-bFGF组及MEBO组大鼠创面组织形态学差异不明显，均可见炎性细胞逐渐减少，新生血管及皮肤附属器逐渐形成，创面组织形态逐渐接近正常，而慢创组大鼠创面组织中炎性细胞消散及新生血管生长较慢，逐渐形成瘢痕组织形态。（2）局部处理第3、7、14天，急创组、慢创组、rb-bFGF组及MEBO组大鼠创面组织中CK14蛋白表达及其mRNA转录水平均呈逐渐升高趋势（P均＜0.05），至局部处理第14天，急创组、rb-bFGF组及MEBO组均升高至空白组水平（P均＞0.05），而慢创组仍低于其它各组（P均＜0.05）。（3）局部处理第3、7、14天，急创组、慢创组、rb-bFGF组及MEBO组大鼠创面组织中CK19蛋白表达及其mRNA转录水平均呈先升高后降低的趋势（P均＜0.05），至局部处理第14天，急创组、rb-bFGF组及MEBO组均降低至空白组水平（P均＞0.05），而慢创组仍高于其它各组（P均＜0.05）。结论 MEBT/MEBO能够促进慢性难愈合创面愈合，且调控创面组织中CK14及CK19的表达水平可能是其部分作用机制。 |
| 英文摘要: |
| Objective To explore the influence of MEBT/ MEBO ( moist exposed burn therapy/moist exposed burn ointment) on the expression levels of CK14 and CK19 in chronic refractory wound tissues of rats. Methods 90 SPF Wistar rats were divided, according to the random number table, into blank group, acute wound group, chronic wound group, rb-bFGF group and MEBO group, with 18 rats in each group. The rats in the blank group were only given back skin preparation. Acute wound models were established on the rats of the acute wound group, and chronic wound models were made on the rats of the chronic wound group, rb-bFGF group and MEBO group. After model establishment, the skin prepa-ration area in the blank group and the wounds in the acute wound group and chronic wound group were managed with moist dressing with saline gauze; the wounds in the rb-bFGF group were treated with bovine basic fibroblast growth factor ( rb-bFGF) gel and the wounds in the MEBO group were treated with MEBO (moist exposed burn ointment). On respective day 3, 7 and 14 of treatment, the skin or wound tissues of rats in each group were taken for Masson staining to observe the histo-morphological differences among them. Western blotting and RT-PCR were performed to compare the expression levels of CK14 and CK19 and their mRNA transcription levels. Results (1) Masson staining results showed that no significant histomorphological differences was observed among the acute wound group, rb-bFGF group and MEBO group, and on the wounds of the three groups, the inflammatory cells gradually decreased, new blood vessels and skin appendages gradually took into form, and the histomorphology of wound tissues gradually turned to normal. In contrast, on the wounds of the chro-nic wound group, the inflammatory cells were dissipated, new blood vessels grew slowly, and scar tissues formed gradually. (2) On day 3, 7 and 14 of treatment, the expression level of CK14 and its mRNA transcription level in the wound tissues of rats in the acute wound group, chronic wound group, rb-bFGF group and MEBO group showed a trend of gradual increase (all P < 0.05). On day 14 of treatment, their levels in the acute wound group, rb-bFGF group and MEBO group increased to the same levels as that in the blank group (all P > 0.05), whereas the levels in the chronic wound group were still lower than that in the other four groups (all P < 0.05). (3) On day 3, 7 and 14 of treatment, the expression level of CK19 and its mRNA transcription level in the wound tissues of rats in the acute wound group, chronic wound group, rb-bFGF group and MEBO group showed a trend of increase followed by decrease (all P < 0.05). On day 14 of treatment, their levels in the acute wound group, rb-bFGF group and MEBO group decreased to that of the blank group ( all P > 0.05), whereas in the chronic wound group, the levels were still higher than other groups ( all P < 0.05). Conclusion MEBT/MEBO can promote the healing of chronic refractory wounds, and regulating the expression levels of CK14 and CK19 in wound tissues may be part of its mechanism of action. |
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