| 何明杰,甘忠贵,郑爱甜,黄 庆,吴标良.高压氧对糖尿病足创面组织中 VEGF、EGF 表达水平的影响[J].中国烧伤创疡杂志,2021,(4):237~242. |
| DOI： |
| 中文关键词: |
| 英文关键词: |
| 基金项目:广西临床重点专科建设项目 (桂卫医发 [2018] 6 号); 广西卫健委自筹经费科研课题 (220180197) |
|
| 摘要点击次数: 1492 |
| 全文下载次数: 4034 |
| 中文摘要: |
| 【摘要】 目的 探讨高压氧对糖尿病足创面组织中血管内皮生长因子 (VEGF)、表皮生长因子 (EGF) 表 达水平的影响? 方法 选取2016 年 3月至 2019 年6月右江民族医学院附属医院收治的48例Wagner 1 ~ 2级糖尿病足患者作为研究对象, 并按照随机区组设计方法将其随机分为研究组和对照组, 每组24例，对照组患者行常规清创换药治疗, 研究组患者在对照组治疗的基础上联合应用高压氧治疗，对比观察两组患者治疗后创面组织形态学及 VEGF、EGF表达水平变化情况? 结果 治疗第 3、7、12 天，对照组患者创面组织水肿逐渐消退、炎症细胞逐渐减少、新生血管逐渐增多但排列紊乱，研究组患者创面组织水肿消退及炎症细胞减少速度明显快于对照组，治疗第3天即可见排列整齐的新生血管及层状分布的肉芽组织；免疫组化检测结果显示，VEGF表达于血管内皮细胞及成纤维细胞胞浆内，EGF表达于血管内皮细胞、成纤维细胞及炎症细胞胞浆内；治疗第 3、7、12天，两组患者创面组织内 VEGF? EGF 表达水平均逐渐升高，除治疗第3天两组患者创面组织内 VEGF 表达水平无显著差异 (t = 1.49, P = 0.33) 外，治疗第 7、12天研究组患者创面组织内VEGF表达水平及各时间点EGF表达水平均明显高于对照组 (VEGF: t = 4.10、4.36，P均< 0.001；EGF: t = 2.63，3.21，4.509， P= 0.048、P = 0.001、P < 0.001)? 结论 高压氧可通过上调创面组织内 VEGF、EGF 的表达水平，促进糖尿病足面组织内新生血管的生成，加快创面愈合。 |
| 英文摘要: |
| 【Abstract】 Objective To investigate the influence of hyperbaric oxygen on the expression of vascular endothelial growth factor (VEGF) and epidermal growth factor (EGF) in diabetic foot wounds. Methods Forty?eight patients withdiabetic foot of Wagner grade Ⅰ and Ⅱ, admitted to the Affiliated Hospital of Youjiang Medical University for Nationalities from March 2016 to June 2019, were selected as study subjects to be divided into a study group and a control group according to the randomized block design, with 24 cases in each group. Patients in the control group were given the conventional debridement and dressing change, whereas patients in the study group were given the hyperbaric oxygen therapy in addition to the conventional debridement and dressing change. The histomorphological changes and the expression of VEGF and EGF after treatment were observed and compared between the two groups. Results On day 3, 7 and 12 of treatment, the wound tissue edema gradually subsided, amount of inflammatory cells gradually decreased, and newly?grown blood vessels increased gradually though arranged in disorder in the control group, while in the study group the speeds of edema disappearance and inflammatory cell decrease were obviously faster than that in the control group, and on day 3 of treatment, wellarranged new blood vessels and stratified granulation tissues were visible. The immunohistochemical test showed that the expression of VEGF was in the cytoplasm of vascular endothelial cells and fibroblasts, and EGF was expressed in the cytoplasm of vascular endothelial cells, fibroblasts and inflammatory cells. On day 3, 7 and 12 of treatment, the expression of VEGF and EGF in the wound tissues of the two groups were all increased gradually, the expression levels of VEGF and EGF in the wound tissues in the study group were all obviously higher than that in the control group respectively on day 7 and 12 of treatment (VEGF: t = 4.810, 4.36, both P < 0.001; EGF: t = 2.963, 3.521, 4.509, P = 0.048,P = 0.001, P < 0.001), except that on day 3 of treatment there was no statistically significant difference between the two groups in terms of the expression of VEGF (t = 1.549, P = 0.133). Conclusion Hyperbaric oxygen can promote the generation of new blood vessels in diabetic foot wound tissues and accelerate wound healing by upregulating the expression of EGF and EGF in wound tissues. |
|
|
|
|
