| 贺佐分,岑丽君,郭文闻,童丹蕾,黄金梅,葛星月,杨雅量,李文武,唐乾利.MEBT/ MEBO对慢性难愈合创面 NF-κB p65、IκBα、IKK及其磷酸化蛋白表达水平的影响[J].中国烧伤创疡杂志,2022,(4):153~161. |
| DOI： |
| 中文关键词: 创疡再生医疗技术 湿润烧伤膏 慢性难愈合创面 核因子 κB p65 NF⁃κB 抑制蛋白 α IκB激酶 |
| 英文关键词:The regenerative medical technology for burns, wounds and ulcers MEBO Chronic refractorywounds Nuclear factor⁃κB (NF⁃κB) p65 Inhibitor⁃κ binding protein α(IκBα) IκB kinase |
| 基金项目:国家自然科学基金面上项目 (81774327); “广西特聘专家” 专项经费资助项目 (桂人才通字 [2019] 13 号); 广西医学高层次领军人才培养 “139” 计划资助项目 (桂卫科教发 [2018] 22 号) |
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| 中文摘要: |
| 【摘要】 目的 探讨创疡再生医疗技术 (MEBT/MEBO) 对慢性难愈合创面组织中核因子 κB (NF-κB) p65?NF-κB 抑制蛋白(IκB)、IκB激酶(IKK) 及其磷酸化蛋白表达水平的影响。 方法 选取 SPF 级雄性 Wistar 大鼠90 只适应性饲养1周后，按照随机数表法将其随机分为空白组、对照组、模型组、MEBO组和贝复新组，每组18 只。空白组大鼠仅做备皮处理，对照组大鼠建立急性创面模型，模型组、MEBO组、贝复新组大鼠建立慢性难愈合创面模型?空白组大鼠备皮处皮肤及对照组、模型组大鼠创面采用生理盐水纱布换药处理，MEBO 组大鼠创面采用湿润烧伤膏 (MEBO) 药纱换药处理，贝复新组大鼠创面采用重组牛碱性成纤维细胞生长因子换药处理,对比各组大鼠干预第3、7、14天创面愈合率、组织病理学变化，以及皮肤/ 创面组织中NF-κB p65、IκBα、IKK及 p-NF-κB p65、p-IκBα、p-IKK 蛋白表达水平。结果 (1)干预第 3、7 天，各组大鼠创面愈合率均无明显差异(P均＞0. 05)；干预第 14 天，模型组大鼠创面愈合率明显低于对照组、MEBO组(P均＜0.05)，其余各组间创面愈合率均无明显差异 (P均＞0.05)。(2)干预第 7 天，MEBO 组、贝复新组大鼠创面组织中胶原纤维量均明显增加，可见大量成纤维细胞和新生毛细血管及少量毛囊结构生成； 干预第14天，MEBO 组、贝复新组大鼠创面组织中胶原纤维量明显多于模型组，可见整齐排列的毛细血管及成熟的毛囊、皮脂腺等组织，而模型组大鼠创面组织中仍可见少量炎症细胞浸润，但已有大量成纤维细胞生成。(3) 干预第7、14天，空白组、对照组、MEBO组大鼠创面组织中 NF-κB p65、IKK、p-NF-κB p65、p-IKK、p-IκBα 蛋白表达水平均明显低于模型组(P均＜0.05)；干预第 7 天，MEBO 组大鼠创面组织中 NF-κB p65、IκBα 蛋白表达水平均明显高于贝复新组 (P均＜0.05)，IKK、p-NF-κB p65、p-IKK、p-IκBα 蛋白表达水平均明显低于贝复新组 (P 均＜0.05)； 干预第 14天，MEBO 组大鼠创面组织中 IKK、IκBα蛋白表达水平均明显低于贝复新组 (P 均＜0.05)。结论 MEBT/ MEBO促进慢性难愈合创面愈合的机制可能与创面组织中 NF-κB p65、IκBα、IKK 及其磷酸化蛋白的表达水平有关。 |
| 英文摘要: |
| 【Abstract】 Objective To investigate the influence of the regenerative medical technology for burns, wounds and ulcers (MEBT/ MEBO) on expression levels of nuclear factor-κB (NF-κB) p65, inhibitor-κ binding protein - nuclear factor of kappa light polypeptide gene enhancer in B-cells inhibitor, alpha (IκBα), IκB kinase ( IKK) and their phosphorylated proteins (p-NF-κB p65, p-IκBα, P-IKK) in chronic refractory wounds. Methods Ninety SPF-bred male Wistar rats were selected and, after adaptive feeding for one week, randomized into five groups: blank group, control group, model group,MEBO group and rb-bFGF group, 18 rats in each group. Only skin preparation was performed on rats in blank group,whereas acute wound models were established on rats in control group, and chronic refractory wound models were made on rats respectively in model group, MEBO group and rb-bFGF group. After model establishment, the prepared skin in blank group and the wounds in control group and model group were managed with dressing change of normal saline gauzes, and the wounds in rb-bFGF group and MEBO group were respectively managed with dressing change of recombinant bovine basic fibroblast growth factor (rb-bFGF) and MEBO. The wound healing rate, histopathological changes and the expression levels of NF-κB p65, IκBα, IKK and p-NF-κB p65, p-IκBα, p-IKK in the skin or wound tissues of rats in each group were compared on day 3, 7 and 14 of intervention. Results (1) No significant difference was observed among the five groups on day 3 and 7 of intervention in terms of wound healing rate (all P＞0.05); on day 14 of intervention, the wound healing rate in model group was significantly lower than that in control group and MEBO group ( both P＜0.05), while no significant difference in wound healing rate among other groups (all P＞0.05). (2) On day 7 of intervention, the amount of collagen fibers in the wound tissues of rats increased significantly, and a large number of fibroblasts, newly born capillaries and few hair follicle structure were generated in MEBO group and rb-bFGF group; on day 14 of intervention, the numbers of collagen fibers in the wound tissues of rats in MEBO group and rb-bFGF group were obviously more than that in model group, with orderly arranged capillaries, mature hair follicles and sebaceous glands visible; In model group, infiltration of a small number of inflammatory cells was still observable in the wound tissues, but there were already a lot of fibroblasts generated. (3)On day 7 and 14 of intervention, the expression levels of NF-κB p65, IKK, p-NF-κB p65, p-IKK and p-IκBα in the skin or wound tissues of rats in blank group, control group and MEBO group were significantly lower than that in model group (all P＜0.05); on day 7 of intervention, the expression levels of NF-κB p65 and IκBα in the wound tissues of rats in MEBO group were significantly higher than that in rb-bFGF group (both P＜0.05), whereas the expression levels of IKK,p-NF-κB p65, p-IKK and p-IκBα were significantly lower than that in rb-bFGF group (all P＜0.05); on day 14 of inter-vention, the expression levels of IKK and IκBα in the wound tissues of rats in MEBO group were obviously lower than that in rb-bFGF group (both P＜0.05). Conclusion The mechanism of MEBT/ MEBO in promoting the healing of chronic refractory wounds may be associated with the expression levels of NF-κB p65, IκBα, IKK and their phosphorylated proteins in wound tissues. |
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