| 赵正阳,田播文,宋均辉,李兴照,余又新,胡德林,孙业祥.不同浓度高渗钠盐溶液对重度烫伤大鼠炎症反应和肝脏氧化损伤的影响[J].中国烧伤创疡杂志,2022,(6):408~412. |
| DOI： |
| 中文关键词: 烧伤 休克 高渗钠盐溶液 肝脏 炎症 氧化应激 |
| 英文关键词:Burns Shock Hypertonic sodium salt solution Liver Inflammation Oxidative stress |
| 基金项目:国家自然科学基金（81272092） |
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| 中文摘要: |
| 【摘要】 目的 探讨不同浓度高渗钠盐溶液 (HS) 对重度烫伤大鼠炎症反应与肝脏氧化应激反应的影响。 方法 选取 SPF 级雌性 SD 大鼠 176 只适应性饲养 1 周后, 按照随机数表法将其随机分为假伤组 (8 只)、LR 组 (24 只)、200HS 组 (24 只)、300HS 组 (24 只)、400HS 组 (24 只)、600HS 组 (24 只)、800HS 组 (24 只)、1000HS 组 (24 只), 其中假伤组大鼠做假伤处理后直接处死, LR 组大鼠建立重度烫伤模型后予以 4 ml/kg 的乳酸钠林格注射液(LR)补液治疗, 200HS组、300HS组、400HS组、600HS组、800HS组、1000HS组大鼠建立重度烫伤模型后分别予以200 mmol/L、300 mmol/L、400 mmol/L、600 mmol/L、800 mmol/L、1000 mmol/L的HS补液治疗, 对比观察各组大鼠血清肿瘤坏死因子-α( TNF-α)、白细胞介素-1β ( IL-1β)、高迁移率族蛋白B1 (HMGB1)水平以及肝脏组织中丙二醛 (MDA)、超氧化物歧化酶(SOD)水平变化情况。结果 烫伤后, 各组大鼠血清 TNF-α、IL-1β水平均随时间的延长呈现先升高后降低的趋势, 血清 HMGB1水平均随时间的延长呈现逐渐升高的趋势, 且不同浓度 HS 组多显著低于LR组、600HS组多显著低于其他浓度 HS 组 (P均 < 0.05); 肝脏组织中 MDA 水平均随时间的延长呈现先升高后降低的趋势, 且不同浓度HS组多显著低于LR组、600HS 组多显著低于其他浓度 HS组(P均 < 0.05)；肝脏组织中 SOD 水平均随时间的延长呈现先降低后升高的趋势，且不同浓度 HS 组多显著高于 LR 组、600HS 组多显著高于其他浓度HS组 (P 均 < 0.05)。结论 与 LR 补液治疗相比, HS 可通过调控 TNF-α? IL-1β等炎症因子释放以及减少肝脏组织MDA累积、促进SOD分泌而减轻烧伤后炎症反应及肝脏氧化应激损伤, 且以600 mmol / LHS的效果最佳。 |
| 英文摘要: |
| 【Abstract】 Objective To study the effects of hypertonic sodium salt solution (HS) at different concentrations on in flammatory response and liver oxidative injury of severely scalded rats. Methods 176 SPF female SD rats were selected and divided, after adaptive feeding for one week, into sham group (n = 8), LR group (n = 24), 200HS group (n = 24), 300HS group (n = 24), 400HS group (n = 24), 600HS group (n = 24), 800HS group (n = 24) and 1000HS group (n = 24) using the random number table. The rats in sham group were sacrificed directly after pseudo wound was made; the rats in LR group were given 4 ml / kg sodium lactate ringer’s injection (LR) after the wound models of severe scalds were established; the rats were given 200 mmol / L, 300 mmol / L, 400 mmol / L, 600 mmol / L, 800 mmol / L and 1000 mmol / L HS respectively in 200HS group, 300HS group, 400HS group, 600HS group, 800HS group and 1000HS group after the wound models of severe scalds were established. The levels of tumor necrosis factor-α (TNF-α), interleukin?1β ( IL?1β), high mobility group protein B1 (HMGB1) in serum and changes of malondialdehyde (MDA) and superoxide dismutase (SOD) in liver tissues of rats were observed and compared among the groups. Results After scalds, the levels of serum TNF?α and IL-1β of rats in each group showed a trend of decrease following increase over time, and the levels of serum HMGB1
showed a gradually increasing trend over time, but their levels in HS groups at different concentrations were mostly significantly lower than that in LR group, and their levels in 600HS group were mostly obviously lower than that in HS groups at other concentrations (all P < 0.05); the MDA levels in liver tissues of rats in each group also showed a trend of decrease following increase over time, and the levels in HS groups at different concentrations were mostly significantly lower than that in LR group, and the levels in 600HS group were mostly markedly lower than that in HS groups at other concentrations (all P < 0.05); the SOD levels in liver tissues of rats in each group presented a trend of increase following decrease over time, and the levels in HS groups at different concentrations were mostly significantly higher compared with LR group, and the levels in 600HS group were mostly markedly higher compared with HS groups at other concentrations ( all P < 0.05). Conclusion Compared with LR fluid replacement, HS can alleviate the post?burn inflammatory responses and liver oxidative stress by regulating the release of inflammatory factors including TNF-α and IL-1β, reducing the accumulation of MDA in liver tissues and promoting the secretion of SOD, especially the HS at 600 mmol / L presenting best effects. |
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