| 李 浩,张 磊,徐 敏.低热损伤对大鼠坐骨神经血神经屏障功能的影响[J].中国烧伤创疡杂志,2022,(6):413~417. |
| DOI： |
| 中文关键词: 低热损伤 坐骨神经 血神经屏障 伊文思蓝 病理变化 |
| 英文关键词:Low temperature injury Ischiadic nerve Blood⁃nerve barrier Evans blue Pathological changes |
| 基金项目:宜宾市重点科技计划项目 (2016SF006) |
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| 中文摘要: |
| 【摘要】 目的 探讨低热损伤对大鼠坐骨神经血神经屏障功能的影响。方法 选取 24 只 SPF 级雄性 Wistar 大鼠适应性饲养 1 周后按照随机数表法随机分为热损伤后 1 d 组、热损伤后 3 d 组和热损伤后 5 d 组, 每组 8 只。所有大鼠均建立右侧坐骨神经低热 (47 ℃ ) 损伤模型, 而左侧坐骨神经作为正常对照; 模型建立后, 热损伤后 1 d 组、热损伤后 3 d 组、热损伤后 5 d组大鼠分别于室温下饲养 23、71、119 h, 而后经尾静脉注射伊文思蓝溶液, 采用比色法对比观察 3 组大鼠两侧坐骨神经内伊文思蓝浓度、甲苯胺蓝染色后光学显微镜下观察伊文思蓝分布情况、醋酸铀-枸橼酸铅染色后透射电子显微镜下观察坐骨神经病理变化情况? 结果 热损伤后 1 d 组大鼠两侧 坐骨神经内伊文思蓝浓度均明显高于热损伤后 3 d 组和热损伤后 5 d 组 (F = 30.880、8.213, P < 0.001、P =0.002), 且热损伤后 1 d 组和热损伤后 3 d 组大鼠热损伤侧坐骨神经内伊文思蓝浓度均明显高于对照侧 ( t =3.926、3.736, P = 0.001、0.002), 而热损伤后 5 d 组大鼠两侧坐骨神经内伊文思蓝浓度无明显差异 ( t = 0.701, P = 0.494)。 3 组大鼠热损伤侧坐骨神经内膜均可见弥漫性红色荧光, 而对照侧伊文思蓝红色荧光仅局限于神经内膜血管腔内, 未渗漏至血管外? 3组大鼠热损伤侧坐骨神经有髓纤维可见雪旺细胞胞浆内空泡形成、髓鞘松散、髓鞘球形成, 轴索空化、内部微丝溶解;神经内膜血管内可见部分紧密连接消失, 红细胞聚集, 内皮细胞出现凋亡; 无髓纤维基本正常? 3 组大鼠对照侧坐骨神经有髓纤维可见少量髓鞘松散,无髓纤维与神经内膜血管基本正常? 结论 47 ℃低热损伤可破坏坐骨神经的血神经屏障功能, 引起神经内膜水肿, 且低热损伤可选择性损伤有髓纤维, 而对无髓纤维损伤极轻。 |
| 英文摘要: |
| 【Abstract】 Objective To investigate the influence of low temperature injury on the blood?nerve barrier function of ischiadic nerves in rats. Methods 24 SPF male Wistar rats were selected, and divided, after adaptive feeding for one week, into group of 1 day after injury (n = 8), group of 3 days after injury (n = 8) and group of 5 days after injury (n = 8) using the random number table. Low temperature injury models (47 ℃ ) were established at right ischiadic nerves in all rats and the left ischiadic nerves were set as control. After the establishment of the models, rats in group of 1 day after injury,group of 3 days after injury, and group of 5 days after injury were fed respectively for 23 h, 71 h, and 119 h at room temperature, and then Evans blue solution were injected into the rats of the three groups through tail vein. Colorimetry was adopted to compare the concentrations of Evans blue solution in the bilateral ischiadic nerves of rats in the three groups, the distribution of Evans blue solution was observed under optical microscope after toluidine blue staining, and the pathological changes of ischiadic nerves were observed under transmission electron microscope after uranyl acetate ? lead citrate staining. Results The concentrations of Evans blue solution in the bilateral ischiadic nerves of rats in group of 1 day after injury were significantly higher respectively compared with group of 3 days after injury and group of 5 days after injury (F = 30.880 and 8.213, P < 0.001, P = 0.002). In group of 1 day after injury and group of 3 days after injury, the concentrations of Evans blue solution in right ischiadic nerves of rats were significantly higher than the left control sides ( t = 3.926 and 3.736, P = 0.001 and 0.002), and no significant difference was observed in the concentration of Evans blue solution in the bilateral ischiadic nerves of rats in group of 5 days after injury (t = 0.701, P = 0.494). Diffuse red fluorescence was visible in the right ischiadic nerve endoneurium in rats of all the three groups, while the red fluorescence of Evans blue was only confined to the endoneurium vascular lumen and did not leak outside in the left control sides. In the myelinated fibers of the right ischiadic nerves of rats in the three groups, vacuoles developed in the cytoplasm of Schwann cells, myelin sheath was loose, myelin balls formed, axonal cavitation was visible, and internal microfilaments were dissolved; Some tight junctions lost, red blood cells aggregated and apoptosis of endothelial cells were observed in endoneurial vessels; and the unmyelinated fibers were basically normal. In contrast, few loose myelin sheath was observed in the myelinated fibers of the left ischiadic nerves of rats in the three groups, and the unmyelinated fibers and endoneurial vessels were basically normal. Conclusion Low temperature injury (47 ℃ ) can destroy the blood?nerve barrier of ischiadic nerves and cause endoneurial edema. Moreover, low temperature injury can selectively damage the myelinated fibers, and bring slight damage to the unmyelinated fibers. |
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