| 刘广星,朱茜颖,曾宏宇,廖秋姣,苏志学,唐 强.创疡再生医疗技术对慢性难愈合创面大鼠血清IL-17A表达水平的影响[J].中国烧伤创疡杂志,2025,(2):85~90. |
| DOI： |
| 中文关键词: 慢性难愈合创面 白细胞介素-17A 重组牛碱性成纤维细胞生长因子 创疡再生医疗技术 |
| 英文关键词:Chronic refractory wound Interleukin-17A Recombinant bovine basic fibroblast growth factor Regenerative medical technology for wounds and ulcers |
| 基金项目:国家自然科学基金 (82160907)；中国红十字基金会徐荣祥再生生命公益基金科研项目 (RXRL2021-03) |
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| 中文摘要: |
| 【摘要】目的探讨创疡再生医疗技术对慢性难愈合创面大鼠血清白细胞介素-17A(IL-17A)表达水平的影响。方法采用随机数表法将100只大鼠随机分为对照组、急创组、慢创组、再生组及贝复新组，每组20只。对照组大鼠备皮后不建立创面模型；急创组大鼠建立急性创面模型，并应用生理盐水治疗；慢创组、再生组及贝复新组大鼠建立慢性难愈合创面模型，并分别应用生理盐水、创疡再生医疗技术、重组牛碱性成纤维细胞生长因子凝胶(简称贝复新)治疗，对比各组大鼠创面愈合情况、组织形态学变化及血清IL-17A表达水平。结果建模后第3、5、7、14天，急创组、再生组、贝复新组大鼠创面愈合率均明显高于慢创组(第3天:q=16.840、11.141、23.322，P均＜0.001；第5天:q=33.802、26.763、33.101，P均＜0.001；第7天:q=51.710、49.384、45.901，P均＜0.001；第14天:q=66.672、63.772、66.963，P均＜0.001)；建模后第3、5天贝复新组大鼠创面愈合率明显高于再生组（q=12.183、6.338，P均＜0.001），而第7、14天两组间无明显差异（q=3.486、3.189，P=0.104、0.151）。建模后第7、14天，慢创组大鼠创面组织内仍可见炎症细胞浸润，而急创组、再生组、贝复新组大鼠创面组织内均可见新生毛细血管生长。建模后第3、5、7、14天，对照组大鼠血清IL-17A水平无明显变化，且除第3天再生组与对照组间无明显差异(q=3.478，P=0.140)外，其余时间急创组、慢创组、再生组、贝复新组均明显高于对照组(第3天:q=8.157、9.462、8.455，P均＜0.001；第5天:q=11.891、16.431、10.490、12.451，P均＜0.001；第7天:q=28.910、42.440、30.230、29.940，P均＜0.001；第14天:q=5.881、9.295、7.688、6.879，P均＜0.001)；建模后第7天，再生组、贝复新组大鼠血清IL-17A水平均明显低于慢创组(q=12.211、12.493，P均＜0.001)；建模后第5、7、14天，再生组与贝复新组间大鼠血清IL-17A水平均无明显差异(q=1.955、0.284、0.809，P=0.645、0.999、0.978)。结论创疡再生医疗技术可通过下调IL-17A的表达水平促进大鼠慢性难愈合创面愈合。 |
| 英文摘要: |
| 【Abstract】 Objective To study the effect of regenerative medical technology for wounds and ulcers on expression level of serum interleukin-17A (IL-17A) in rats with chronic refractory wounds. Methods 100 rats were randomly divided into five groups using a random number table: control group, acute wound group, chronic wound group, regeneration group, and rb-bFGF group, with 20 rats in each group. Rats in the control group received skin preparation without wound modeling, while rats in the acute wound group received acute wound modeling and treatment with saline. Rats in the chronic wound group, regeneration group, and rb-bFGF group received chronic refractory wound modeling and were treated with saline, regeneration medical technology for wounds and ulcers, and recombinant bovine basic fibroblast growth factor (rb-bFGF) gel, respectively. Wound healing, histopathological changes, and the expression levels of serum IL-17A were compared among the groups. Results On days 3, 5, 7 and 14 after modeling, the wound healing rates of rats in the acute wound group, regeneration group, and rb-bFGF group were significantly higher than those in the chronic wound group (Day3: q = 16.840, 11.141 and 23.322, all P<0.001; Day 5: q = 33.802, 26.763 and 33.101, all P<0.001; Day 7: q =51.710, 49.384 and 45.901, all P<0.001; Day 14: q = 66.672, 63.772 and 66.963, all P<0.001). On days 3 and 5 after modeling, the wound healing rate of rats in the rb-bFGF group was significantly higher than that of the regeneration group(q = 12.183 and 6.338, both P<0.001), while there were no significant differences between the two groups on days 7 and 14 (q = 3.486 and 3.189, P= 0.104 and 0.151). On days 7 and 14, inflammatory cell infiltration was still observed in the chronic wound group, while new capillary growth was observed in the acute wound group, regeneration group, and rb-bFGF group. On days 3, 5, 7 and 14 after modeling, there were no significant changes in serum IL-17A levels in the control group. Except for day 3, where no significant difference was found between the regeneration group and the control group (q =3.478, P= 0.140), the serum IL-17A levels were significantly higher in the acute wound group, chronic wound group,regeneration group, and rb-bFGF group compared to the control group (Day 3: q = 8.157, 9.462 and 8.455, all P<0.001; Day 5: q = 11.891, 16.431, 10.490 and 12.451, all P<0.001; Day 7: q = 28.910, 42.440, 30.230 and 29.940, all P<0.001; Day 14: q = 5.881, 9.295, 7.688 and 6.879, all P<0.001). On day 7 after modeling, the serum IL-17A levels in the regeneration group and the rb-bFGF group were significantly lower than in the chronic wound group ( q = 12.211 and 12.493, both P<0.001). On days 5, 7 and 14 after modeling, there were no significant differences in the serum IL-17A levels of rats between the regeneration group and the rb-bFGF group (q = 1.955, 0.284 and 0.809, P= 0.645, 0.999 and 0.978). Conclusion The regenerative medical technology for wounds and ulcers can promote the healing of chronic refractory wounds in rats by down-regulating the expression level of IL-17A. |
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